Biology and disease are heterogenous. It is hard to visualise, quantify, and qualify this heterogeneity at the structural and ultra-structural levels, within single proteins and protein assemblies, and in human and cellular samples. We are a research lab at the University of St Andrews developing new instrumentation, assays, and software to push single molecule and super resolution microscopy towards structural imaging one-molecule-at-a-time. Together with collaborators, we aim to democratise these technologies and use them to study the molecular architecture of cellular complexes, from the Kinetochore to pore-forming proteins, and human diseases, from neurodegenerative to immune disorders in living human samples.